Ontology highlight
ABSTRACT:
SUBMITTER: Abdi K
PROVIDER: S-EPMC6215252 | biostudies-literature | 2018 Nov
REPOSITORIES: biostudies-literature
Abdi Kaveh K Laky Karen K Padhan Kartika K Petrovas Constantinos C Skinner Jeff J Kabat Juraj J Dorward David W DW Brzostowski Joseph J Long Eric O EO Trinchieri Giorgio G Varma Rajat R
Journal of immunology (Baltimore, Md. : 1950) 20181012 10
Early secretion of IL-12 by mouse dendritic cells (DCs) instructs T cells to make IFN-γ. However, only activated, but not naive T cells are able to license DCs for IL-12 production. We hypothesized that it might be due to different levels of CD40L expression on the surface of these cells, as CD40 signals are required for IL-12 production. Using quantitative cell-free systems incorporating CD40L in lipid bilayers combined with total internal reflection fluorescence microscopy and flow cytometry, ...[more]