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Efficient immunoaffinity chromatography of lymphocytes directly from whole blood.

ABSTRACT: We show that defined lymphocytes can be rapidly purified by immunoaffinity chromatography starting directly from whole blood. The method relies on low-affinity Fab-fragments attached to a column-matrix combined with the reversible Strep-tag technology. Compared to established cell enrichment protocols, the Strep-tag affinity chromatography of cells is independent of erythrocyte lysis or centrifugation steps, allowing for simple cell-enrichment with good yields, high purities, and excellent functionality of purified cells.

SUBMITTER: Mohr F

PROVIDER: S-EPMC6233198 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature

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Efficient immunoaffinity chromatography of lymphocytes directly from whole blood.

Mohr Fabian F Przibilla Sabine S Leonhardt Franziska F Stemberger Christian C Dreher Stefan S Müller Thomas R TR Fräßle Simon P SP Schmidt Georg P GP Kiene Marie-Luise ML Stadler Herbert H Busch Dirk H DH

Scientific reports 20181113 1

We show that defined lymphocytes can be rapidly purified by immunoaffinity chromatography starting directly from whole blood. The method relies on low-affinity Fab-fragments attached to a column-matrix combined with the reversible Strep-tag technology. Compared to established cell enrichment protocols, the Strep-tag affinity chromatography of cells is independent of erythrocyte lysis or centrifugation steps, allowing for simple cell-enrichment with good yields, high purities, and excellent funct ...[more]

PMID: 30425259

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