Efficient immunoaffinity chromatography of lymphocytes directly from whole blood.
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ABSTRACT:
SUBMITTER: Mohr F
PROVIDER: S-EPMC6233198 | biostudies-literature | 2018 Nov
REPOSITORIES: biostudies-literature
Publications
Efficient immunoaffinity chromatography of lymphocytes directly from whole blood.
Mohr Fabian F Przibilla Sabine S Leonhardt Franziska F Stemberger Christian C Dreher Stefan S Müller Thomas R TR Fräßle Simon P SP Schmidt Georg P GP Kiene Marie-Luise ML Stadler Herbert H Busch Dirk H DH
Scientific reports 20181113 1
We show that defined lymphocytes can be rapidly purified by immunoaffinity chromatography starting directly from whole blood. The method relies on low-affinity Fab-fragments attached to a column-matrix combined with the reversible Strep-tag technology. Compared to established cell enrichment protocols, the Strep-tag affinity chromatography of cells is independent of erythrocyte lysis or centrifugation steps, allowing for simple cell-enrichment with good yields, high purities, and excellent funct ...[more]
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