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A Catalytic and Selective Scissoring Molecular Tool for Quadruplex Nucleic Acids.

ABSTRACT: A copper complex embedded in the structure of a water-soluble naphthalene diimide has been designed to bind and cleave G-quadruplex DNA. We describe the properties of this ligand, including its catalytic activity in the generation of ROS. FRET melting, CD, NMR, gel sequencing, and mass spectrometry experiments highlight a unique and unexpected selectivity in cleaving G-quadruplex sequences. This selectivity relies both on the binding affinity and structural features of the targeted G-quadruplexes.

SUBMITTER: Nadai M

PROVIDER: S-EPMC6242190 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature

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A Catalytic and Selective Scissoring Molecular Tool for Quadruplex Nucleic Acids.

Nadai Matteo M Doria Filippo F Scalabrin Matteo M Pirota Valentina V Grande Vincenzo V Bergamaschi Greta G Amendola Valeria V Winnerdy Fernaldo Richtia FR Phan Anh Tuân AT Richter Sara N SN Freccero Mauro M

Journal of the American Chemical Society 20181029 44

A copper complex embedded in the structure of a water-soluble naphthalene diimide has been designed to bind and cleave G-quadruplex DNA. We describe the properties of this ligand, including its catalytic activity in the generation of ROS. FRET melting, CD, NMR, gel sequencing, and mass spectrometry experiments highlight a unique and unexpected selectivity in cleaving G-quadruplex sequences. This selectivity relies both on the binding affinity and structural features of the targeted G-quadruplexe ...[more]

PMID: 30351011

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