Ontology highlight
ABSTRACT:
SUBMITTER: Giddens JP
PROVIDER: S-EPMC6255169 | biostudies-literature | 2018 Nov
REPOSITORIES: biostudies-literature
Giddens John P JP Lomino Joseph V JV DiLillo David J DJ Ravetch Jeffrey V JV Wang Lai-Xi LX
Proceedings of the National Academy of Sciences of the United States of America 20181105 47
The N-glycans attached to the Fab and Fc domains play distinct roles in modulating the functions of antibodies. However, posttranslational site-selective modifications of glycans in antibodies and other multiply glycosylated proteins remain a challenging task. Here, we report a chemoenzymatic method that permits independent manipulation of the Fab and Fc N-glycans, using cetuximab as a model therapeutic monoclonal antibody. Taking advantage of the substrate specificity of three endoglycosidases ...[more]