Ontology highlight
ABSTRACT:
SUBMITTER: Toonstra C
PROVIDER: S-EPMC5705187 | biostudies-literature | 2016 Aug
REPOSITORIES: biostudies-literature
The Journal of organic chemistry 20160707 15
A convergent chemoenzymatic approach for sequential installation of different N-glycans in a polypeptide is described. The method includes introduction of distinguishably protected GlcNAc-Asn building blocks during automated solid phase peptide synthesis (SPPS), followed by orthogonal deprotection of the GlcNAc primers and site-selective sequential extension of the sugar chains through glycosynthase-catalyzed transglycosylation reactions. It was observed that the protecting groups on one neighbo ...[more]