Single-Cell Analysis of Quiescent HIV Infection Reveals Host Transcriptional Profiles that Regulate Proviral Latency.
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ABSTRACT:
SUBMITTER: Bradley T
PROVIDER: S-EPMC6258175 | biostudies-literature | 2018 Oct
REPOSITORIES: biostudies-literature
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Single-Cell Analysis of Quiescent HIV Infection Reveals Host Transcriptional Profiles that Regulate Proviral Latency.
Bradley Todd T Ferrari Guido G Haynes Barton F BF Margolis David M DM Browne Edward P EP
Cell reports 20181001 1
A detailed understanding of the mechanisms that establish or maintain the latent reservoir of HIV will guide approaches to eliminate persistent infection. We used a cell line and primary cell models of HIV latency to investigate viral RNA (vRNA) expression and the role of the host transcriptome using single-cell approaches. Single-cell vRNA quantitation identified distinct populations of cells expressing various levels of vRNA, including completely silent populations. Strikingly, single-cell RNA ...[more]
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