Ontology highlight
ABSTRACT:
SUBMITTER: Hans S
PROVIDER: S-EPMC7893016 | biostudies-literature | 2021 Feb
REPOSITORIES: biostudies-literature
Hans Stefan S Zöller Daniela D Hammer Juliane J Stucke Johanna J Spieß Sandra S Kesavan Gokul G Kroehne Volker V Eguiguren Juan Sebastian JS Ezhkova Diana D Petzold Andreas A Dahl Andreas A Brand Michael M
Nature communications 20210218 1
Conditional gene inactivation is a powerful tool to determine gene function when constitutive mutations result in detrimental effects. The most commonly used technique to achieve conditional gene inactivation employs the Cre/loxP system and its ability to delete DNA sequences flanked by two loxP sites. However, targeting a gene with two loxP sites is time and labor consuming. Here, we show Cre-Controlled CRISPR (3C) mutagenesis to circumvent these issues. 3C relies on gRNA and Cre-dependent Cas9 ...[more]