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Human herpesvirus 8 DNA detection and variant analysis in patients with multiple sclerosis.

ABSTRACT: Several studies reported a complex interplay between viral infections and neural cells leading to multiple sclerosis. A role for some viral infections has been proposed in MS. In this study, DNA sequences of human herpesvirus 8 (HHV-8) were searched in the peripheral blood of 54 patients with multiple sclerosis and 130 healthy subjects using nested-PCR assay to amplify ORF26 locus. Furthermore, HHV-8 positive samples were subjected to a nested-PCR to amplify K1 gene of HHV-8 followed by direct nucleotide sequencing. HHV-8 genome was detected in 18.5% (10/54) and 3% (4/130) of MS patients and controls, respectively, and the difference reached statistically significant level (P?=?0.0017). Genotyping analysis revealed that genotype C was common (88.9%) in all study subjects, followed by genotype A. Our results showed higher detection of HHV-8 DNA in MS patients than control group.

SUBMITTER: Marashi SM

PROVIDER: S-EPMC6261899 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature

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Human herpesvirus 8 DNA detection and variant analysis in patients with multiple sclerosis.

Marashi Sayed Mahdi SM Mostafa Aliehossadat A Shoja Zabihollah Z Nejati Ahmad A Shahmahmoodi Shohreh S Mollaei-Kandelous Yaghoub Y Sahraian Mohammad Ali MA Jalilvand Somayeh S

Virusdisease 20180810 4

Several studies reported a complex interplay between viral infections and neural cells leading to multiple sclerosis. A role for some viral infections has been proposed in MS. In this study, DNA sequences of human herpesvirus 8 (HHV-8) were searched in the peripheral blood of 54 patients with multiple sclerosis and 130 healthy subjects using nested-PCR assay to amplify ORF26 locus. Furthermore, HHV-8 positive samples were subjected to a nested-PCR to amplify K1 gene of HHV-8 followed by direct n ...[more]

PMID: 30539059

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Dataset Information

Human herpesvirus 8 DNA detection and variant analysis in patients with multiple sclerosis.

Publications

Human herpesvirus 8 DNA detection and variant analysis in patients with multiple sclerosis.

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