Ontology highlight
ABSTRACT:
SUBMITTER: Yang Y
PROVIDER: S-EPMC6270401 | biostudies-literature | 2013 Oct
REPOSITORIES: biostudies-literature
Molecules (Basel, Switzerland) 20131011 10
Activity-based protein profiling uses chemical probes that covalently attach to active enzyme targets. Probes with conventional tags have disadvantages, such as limited cell permeability or steric hindrance around the reactive group. A tandem labeling strategy with click chemistry is now widely used to study enzyme targets in situ and in vivo. Herein, the probes are reacted in live cells, whereas the ensuing detection by click chemistry takes place in cell lysates. We here make a comparison of t ...[more]