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Tracking electrons in biological macromolecules: from ensemble to single molecule.

ABSTRACT: Nature utilizes oxido-reductases to cater to the energy demands of most biochemical processes in respiratory species. Oxido-reductases are capable of meeting this challenge by utilizing redox active sites, often containing transition metal ions, which facilitate movement and relocation of electrons/protons to create a potential gradient that is used to energize redox reactions. There has been a consistent struggle by researchers to estimate the electron transfer rate constants in physiologically relevant processes. This review provides a brief background on the measurements of electron transfer rates in biological molecules, in particular Cu-containing enzymes, and highlights the recent advances in monitoring these electron transfer events at the single molecule level or better to say, at the individual event level.

SUBMITTER: Tabares LC

PROVIDER: S-EPMC6271485 | biostudies-literature | 2014 Aug

REPOSITORIES: biostudies-literature

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Tracking electrons in biological macromolecules: from ensemble to single molecule.

Tabares Leandro C LC Gupta Ankur A Aartsma Thijs J TJ Canters Gerard W GW

Molecules (Basel, Switzerland) 20140806 8

Nature utilizes oxido-reductases to cater to the energy demands of most biochemical processes in respiratory species. Oxido-reductases are capable of meeting this challenge by utilizing redox active sites, often containing transition metal ions, which facilitate movement and relocation of electrons/protons to create a potential gradient that is used to energize redox reactions. There has been a consistent struggle by researchers to estimate the electron transfer rate constants in physiologically ...[more]

PMID: 25102116

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