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Synthesis of Phospholipid-Protein Conjugates as New Antigens for Autoimmune Antibodies.

ABSTRACT: Copper(I)-catalyzed azide-alkyne cycloaddition, or CuAAC click chemistry, is an efficient method for bioconjugation aiming at chemical and biological applications. Herein, we demonstrate how the CuAAC method can provide novel phospholipid-protein conjugates with a high potential for the diagnostics and therapy of autoimmune conditions. In doing this, we, for the first time, covalently bind via 1,2,3-triazole linker biologically complementary molecules, namely phosphoethanol amine with human ?2-glycoprotein I and prothrombin. The resulting phospholipid-protein conjugates show high binding affinity and specificity for the autoimmune antibodies against autoimmune complexes. Thus, the development of this work might become a milestone in further diagnostics and therapy of autoimmune diseases that involve the production of autoantibodies against the aforementioned phospholipids and proteins, such as antiphospholipid syndrome and systemic lupus erythematosus.

SUBMITTER: Maity A

PROVIDER: S-EPMC6272759 | biostudies-literature | 2015 Jun

REPOSITORIES: biostudies-literature

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Synthesis of Phospholipid-Protein Conjugates as New Antigens for Autoimmune Antibodies.

Maity Arindam A Macaubas Claudia C Mellins Elizabeth E Astakhova Kira K

Molecules (Basel, Switzerland) 20150603 6

Copper(I)-catalyzed azide-alkyne cycloaddition, or CuAAC click chemistry, is an efficient method for bioconjugation aiming at chemical and biological applications. Herein, we demonstrate how the CuAAC method can provide novel phospholipid-protein conjugates with a high potential for the diagnostics and therapy of autoimmune conditions. In doing this, we, for the first time, covalently bind via 1,2,3-triazole linker biologically complementary molecules, namely phosphoethanol amine with human β2-g ...[more]

PMID: 26046322

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