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ABSTRACT: Background
Several mechanisms of action have been proposed to explain the apparent antineoplastic functions of metformin, many of which are observed at high concentrations that may not be reflective of achievable tissue concentrations. We propose that metformin at low concentrations functions to inhibit ROS production and inflammatory signaling in breast cancer, thereby reducing metastasis.Methods
Using the highly invasive MDA-MB-231 breast carcinoma model, we ascertained the impact of metformin on cell viability by DNA content analysis and fluorescent dye exclusion. Migration and invasion assays were performed using a modified Boyden chamber assay and metastasis was ascertained using the chorioallantoic membrane (CAM) assay. PGE2 production was measured by Enzyme-Linked Immunosorbent Assay (ELISA). COX2 and ICAM1 levels were determined by flow cytometry immunoassay.Results
Metformin acutely decreased cell viability and caused G2 cell cycle arrest only at high concentrations (10 mM). At 100 µM, however, metformin reduced ICAM1 and COX2 expression, as well as reduced PGE2 production and endogenous mitochondrial ROS production while failing to significantly impact cell viability. Consequently, metformin inhibited migration, invasion in vitro and PGE2-dependent metastasis in CAM assays.Conclusion
At pharmacologically achievable concentrations, metformin does not drastically impact cell viability, but inhibits inflammatory signaling and metastatic progression in breast cancer cells.
SUBMITTER: Schexnayder C
PROVIDER: S-EPMC6274682 | biostudies-literature | 2018 Nov
REPOSITORIES: biostudies-literature
Schexnayder Chandler C Broussard Kiera K Onuaguluchi Demitrius D Poché Anthony A Ismail Moamen M McAtee LeFontae L Llopis Shawn S Keizerweerd Amber A McFerrin Harris H Williams Christopher C
International journal of molecular sciences 20181121 11
<h4>Background</h4>Several mechanisms of action have been proposed to explain the apparent antineoplastic functions of metformin, many of which are observed at high concentrations that may not be reflective of achievable tissue concentrations. We propose that metformin at low concentrations functions to inhibit ROS production and inflammatory signaling in breast cancer, thereby reducing metastasis.<h4>Methods</h4>Using the highly invasive MDA-MB-231 breast carcinoma model, we ascertained the imp ...[more]