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Amikacin Suppresses Human Breast Cancer Cell MDA-MB-231 Migration and Invasion.

ABSTRACT: (1) Background: Amikacin is an aminoglycoside antibiotic used for treating gram-negative bacterial infections in cancer patients. In this study, our aims are to investigate the migratory inhibition effects of amikacin in human MDA-MB-231 cells. (2) Methods: We used a wound-healing assay, trans-well analysis, Western blotting, immunostaining and siRNA knockdown approaches to investigate how amikacin influenced MDA-MB-231 cell migration and invasion. (3) Results: Wound healing showed that the MDA-MB-231 cell migration rates decreased to 44.4% in the presence of amikacin. Trans-well analysis showed that amikacin treatment led to invasion inhibition. Western blotting demonstrated that amikacin induced thioredoxin-interacting protein (TXNIP) up-regulation. TXNIP was knocked down using siRNA in MDA-MB-231 cell. Using immunostaining analysis, we found that inhibition of TXNIP expression led to MDA-MB-231 pseudopodia extension; however, amikacin treatment attenuated the cell extension formation. (4) Conclusions: We observed inhibition of migration and invasion in MDA-MB-231 cells treated with amikacin. This suggests inhibition might be mediated by up-regulation of TXNIP.

SUBMITTER: Wang YH

PROVIDER: S-EPMC7712503 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature

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Amikacin Suppresses Human Breast Cancer Cell MDA-MB-231 Migration and Invasion.

Wang Yun-Hsin YH Chen Yau-Hung YH Shen Wen-Hao WH

Toxics 20201120 4

(1) Background: Amikacin is an aminoglycoside antibiotic used for treating gram-negative bacterial infections in cancer patients. In this study, our aims are to investigate the migratory inhibition effects of amikacin in human MDA-MB-231 cells. (2) Methods: We used a wound-healing assay, trans-well analysis, Western blotting, immunostaining and siRNA knockdown approaches to investigate how amikacin influenced MDA-MB-231 cell migration and invasion. (3) Results: Wound healing showed that the MDA- ...[more]

PMID: 33233497

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Dataset Information

Amikacin Suppresses Human Breast Cancer Cell MDA-MB-231 Migration and Invasion.

Publications

Amikacin Suppresses Human Breast Cancer Cell MDA-MB-231 Migration and Invasion.

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