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An effective method of RNA extraction from bacteria refractory to disruption, including mycobacteria.

ABSTRACT: A high yield, rapid and simple procedure is described for extracting RNA from mycobacteria and other micro-organisms refractory to disruption. The method yielded 20 microg RNA/109 Mycobacterium bovis BCG, more than 10 times greater than our previous method. Intact full length hsp 70 (dnaK) mRNA was detected by northern blotting and quantitated after heat shock by slot blot hybridisation.

SUBMITTER: Mangan JA

PROVIDER: S-EPMC146465 | biostudies-other | 1997 Feb

REPOSITORIES: biostudies-other

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An effective method of RNA extraction from bacteria refractory to disruption, including mycobacteria.

Mangan J A JA Sole K M KM Mitchison D A DA Butcher P D PD

Nucleic acids research 19970201 3

A high yield, rapid and simple procedure is described for extracting RNA from mycobacteria and other micro-organisms refractory to disruption. The method yielded 20 microg RNA/109 Mycobacterium bovis BCG, more than 10 times greater than our previous method. Intact full length hsp 70 (dnaK) mRNA was detected by northern blotting and quantitated after heat shock by slot blot hybridisation. ...[more]

PMID: 9016612

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