Identifying insertion mutations by whole-genome sequencing.
Ontology highlight
ABSTRACT:
SUBMITTER: Smith HE
PROVIDER: S-EPMC6277054 | biostudies-literature | 2011 Feb
REPOSITORIES: biostudies-literature
Publications
Identifying insertion mutations by whole-genome sequencing.
BioTechniques 20110201 2
Insertion mutagenesis via mobile genetic element is a common technique for the analysis of gene function in model organisms. Next-generation sequencing offers an attractive approach for localizing the site of insertion, but alignment-based mapping of mobile genetic elements is challenging. A computational method for identifying insertion sites is reported herein. The technique was validated by mapping transposons in both bacterial and nematode species. The approach should be extensible to other ...[more]
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