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A Dectin-1-Caspase-8 Pathway Licenses Canonical Caspase-1 Inflammasome Activation and Interleukin-1? Release in Response to a Pathogenic Fungus.


ABSTRACT: Background:Paracoccidioides brasiliensis is equipped with an arsenal of virulence factors that are crucial for causing infection. Our group previously defined the NLRP3 inflammasome as a mediator of P brasiliensis-induced cell damage recognition and induction of effective Th1 immune responses. However, deficiency of caspase-1 only partially reduced interleukin (IL)-1? levels. Methods:In this study, using chemical inhibitors as well as genetically modified mice, we identify an additional pathway for IL-1? production in response to P brasiliensis infection. Results:Paracoccidioides brasiliensis initiated caspase-8-mediated IL-1? production, an event that was necessary for transcriptional priming and posttranslational processing of pro-IL-1?. Caspase-8 synergizes with the canonical NLRP3 inflammasome pathway to control caspase-1 processing and IL-1? maturation, providing a regulatory role for caspase-8 in host resistance to in vivo P brasiliensis infection. Conclusions:Taken together, these findings revealed an important role for caspase-8 in the innate immune response of host cells to P brasiliensis infection, demonstrating a connected network between noncanonical and canonical inflammasomes to coordinate IL-1? production during fungal challenge.

SUBMITTER: Ketelut-Carneiro N 

PROVIDER: S-EPMC6279136 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature

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A Dectin-1-Caspase-8 Pathway Licenses Canonical Caspase-1 Inflammasome Activation and Interleukin-1β Release in Response to a Pathogenic Fungus.

Ketelut-Carneiro Natália N   Ghosh Sreya S   Levitz Stuart M SM   Fitzgerald Katherine A KA   da Silva João Santana JS  

The Journal of infectious diseases 20180101 2


<h4>Background</h4>Paracoccidioides brasiliensis is equipped with an arsenal of virulence factors that are crucial for causing infection. Our group previously defined the NLRP3 inflammasome as a mediator of P brasiliensis-induced cell damage recognition and induction of effective Th1 immune responses. However, deficiency of caspase-1 only partially reduced interleukin (IL)-1β levels.<h4>Methods</h4>In this study, using chemical inhibitors as well as genetically modified mice, we identify an addi  ...[more]

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