Ontology highlight
ABSTRACT:
SUBMITTER: Liu-Shin LP
PROVIDER: S-EPMC6284598 | biostudies-literature | 2018 Nov-Dec
REPOSITORIES: biostudies-literature
Liu-Shin Lily Pei-Yao LP Fung Adam A Malhotra Arun A Ratnaswamy Gayathri G
mAbs 20181019 8
Antibody-drug conjugates (ADCs) that are formed using thiol-maleimide chemistry are commonly produced by reactions that occur at or above neutral pHs. Alkaline environments can promote disulfide bond scrambling, and may result in the reconfiguration of interchain disulfide bonds in IgG antibodies, particularly in the IgG2 and IgG4 subclasses. IgG2-A and IgG2-B antibodies generated under basic conditions yielded ADCs with comparable average drug-to-antibody ratios and conjugate distributions. In ...[more]