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Characterization and functional biology of the soybean aleurone layer.

ABSTRACT: BACKGROUND:Soybean is a globally important oil seed crop. Both the high protein and oil content of soybean seeds make this crop a lucrative commodity. As in higher eukaryotic species with available genomes, the functional annotation of most of soybean's genes still remains to be investigated. A major hurdle in the functional genomics of soybean is a rapid method to test gene constructs before embarking on stable transformation experiments. RESULTS:In this paper we describe the morphology and composition of the persistent single-cell aleurone layer that derives from the endosperm of developing soybean seeds. Its composition compared to cotyledonary tissue indicates the aleurone layer plays a role in both abiotic and biotic stress. The potential utility as the aleurone layer as a transient expression system in soybean was shown. As a near transparent single-cell layer it can be used as a transient expression system to study transgene expression and inter- and intra-cellular targeting as it is amenable to microscopic techniques. CONCLUSION:The transparent single cell aleurone layer was shown to be compositionally comparable to cotyledonary tissue in soybean with an enrichment in oxidative response proteins and shown to be a potential transient expression platform.

SUBMITTER: Schmidt MA

PROVIDER: S-EPMC6293662 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature

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Characterization and functional biology of the soybean aleurone layer.

Schmidt Monica A MA Herman Eliot M EM

BMC plant biology 20181213 1

<h4>Background</h4>Soybean is a globally important oil seed crop. Both the high protein and oil content of soybean seeds make this crop a lucrative commodity. As in higher eukaryotic species with available genomes, the functional annotation of most of soybean's genes still remains to be investigated. A major hurdle in the functional genomics of soybean is a rapid method to test gene constructs before embarking on stable transformation experiments.<h4>Results</h4>In this paper we describe the mor ...[more]

PMID: 30545296

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Characterization and functional biology of the soybean aleurone layer.

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Characterization and functional biology of the soybean aleurone layer.

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