Ontology highlight
ABSTRACT:
SUBMITTER: Wang J
PROVIDER: S-EPMC6301793 | biostudies-literature | 2018 Nov
REPOSITORIES: biostudies-literature
Wang Jiyu J Zhou Jie J Yang Qidi Q Grayhack Elizabeth J EJ
eLife 20181122
Reading frame maintenance is critical for accurate translation. We show that the conserved eukaryotic/archaeal protein Mbf1 acts with ribosomal proteins Rps3/uS3 and eukaryotic Asc1/RACK1 to prevent frameshifting at inhibitory CGA-CGA codon pairs in the yeast <i>Saccharomyces cerevisiae</i>. Mutations in <i>RPS3</i> that allow frameshifting implicate eukaryotic conserved residues near the mRNA entry site. Mbf1 and Rps3 cooperate to maintain the reading frame of stalled ribosomes, while Asc1 also ...[more]