Ontology highlight
ABSTRACT:
SUBMITTER: Farnung L
PROVIDER: S-EPMC6303367 | biostudies-literature | 2018 Dec
REPOSITORIES: biostudies-literature
Nature communications 20181221 1
Transcription of eukaryotic protein-coding genes requires passage of RNA polymerase II (Pol II) through nucleosomes, but it is unclear how this is achieved. Here we report the cryo-EM structure of transcribing Saccharomyces cerevisiae Pol II engaged with a downstream nucleosome core particle at an overall resolution of 4.4 Å. Pol II and the nucleosome are observed in a defined relative orientation that is not predicted. Pol II contacts both sides of the nucleosome dyad using its clamp head and l ...[more]