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Monotopic Membrane Proteins Join the Fold.

ABSTRACT: Monotopic membrane proteins, classified by topology, are proteins that embed into a single face of the membrane. These proteins are generally underrepresented in the Protein Data Bank (PDB), but the past decade of research has revealed new examples that allow the description of generalizable features. This Opinion article summarizes shared characteristics including oligomerization states, modes of membrane association, mechanisms of interaction with hydrophobic or amphiphilic substrates, and homology to soluble folds. We also discuss how associations of monotopic enzymes in pathways can be used to promote substrate specificity and product composition. These examples highlight the challenges in structure determination specific to this class of proteins, but also the promise of new understanding from future study of these proteins that reside at the interface.

SUBMITTER: Allen KN

PROVIDER: S-EPMC6309722 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature

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Monotopic Membrane Proteins Join the Fold.

Allen Karen N KN Entova Sonya S Ray Leah C LC Imperiali Barbara B

Trends in biochemical sciences 20181015 1

Monotopic membrane proteins, classified by topology, are proteins that embed into a single face of the membrane. These proteins are generally underrepresented in the Protein Data Bank (PDB), but the past decade of research has revealed new examples that allow the description of generalizable features. This Opinion article summarizes shared characteristics including oligomerization states, modes of membrane association, mechanisms of interaction with hydrophobic or amphiphilic substrates, and hom ...[more]

PMID: 30337134

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