Ontology highlight
ABSTRACT:
SUBMITTER: Schoneberg J
PROVIDER: S-EPMC6309985 | biostudies-literature | 2018 Dec
REPOSITORIES: biostudies-literature
Science (New York, N.Y.) 20181201 6421
The endosomal sorting complexes required for transport (ESCRTs) catalyze reverse-topology scission from the inner face of membrane necks in HIV budding, multivesicular endosome biogenesis, cytokinesis, and other pathways. We encapsulated ESCRT-III subunits Snf7, Vps24, and Vps2 and the AAA+ ATPase (adenosine triphosphatase) Vps4 in giant vesicles from which membrane nanotubes reflecting the correct topology of scission could be pulled. Upon ATP release by photo-uncaging, this system generated fo ...[more]