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Efficient and precise editing of endogenous transcripts with SNAP-tagged ADARs.

ABSTRACT: Molecular tools that target RNA at specific sites allow recoding of RNA information and processing. SNAP-tagged deaminases guided by a chemically stabilized guide RNA can edit targeted adenosine to inosine in several endogenous transcripts simultaneously, with high efficiency (up to 90%), high potency, sufficient editing duration, and high precision. We used adenosine deaminases acting on RNA (ADARs) fused to SNAP-tag for the efficient and concurrent editing of two disease-relevant signaling transcripts, KRAS and STAT1. We also demonstrate improved performance compared with that of the recently described Cas13b-ADAR.

SUBMITTER: Vogel P

PROVIDER: S-EPMC6322650 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature

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Efficient and precise editing of endogenous transcripts with SNAP-tagged ADARs.

Vogel Paul P Moschref Matin M Li Qin Q Merkle Tobias T Selvasaravanan Karthika D KD Li Jin Billy JB Stafforst Thorsten T

Nature methods 20180702 7

Molecular tools that target RNA at specific sites allow recoding of RNA information and processing. SNAP-tagged deaminases guided by a chemically stabilized guide RNA can edit targeted adenosine to inosine in several endogenous transcripts simultaneously, with high efficiency (up to 90%), high potency, sufficient editing duration, and high precision. We used adenosine deaminases acting on RNA (ADARs) fused to SNAP-tag for the efficient and concurrent editing of two disease-relevant signaling tra ...[more]

PMID: 29967493

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