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The Study of Association Between Polymorphism of TNF-? Gene's Promoter Region and Recurrent Pregnancy Loss.

ABSTRACT: Background:According to the literature review, polymorphisms of tumor necrosis factor alpha's (TNF-?) promoter region are probably the genetic risk factors of recurrent pregnancy loss. This study has investigated five single nucleotide polymorphisms in the TNF-? gene's promoter region to evaluate their relationship with recurrent pregnancy loss disorder. Methods:Blood samples were taken from 65 women with recurrent pregnancy loss (Case group) and 65 healthy women with a history of successful pregnancy (Control group). Polymerase chain reaction with high resolution melting (PCR-HRM) analysis was done to determine the promoter region of -308G/A, -850T/C, -238G/A, -1031T/C and -863A/C TNF-? polymorphisms. The data were assessed using logistic regression models. P values less than 0.05 were considered statistically significant. Results:Significant associations were found between recurrent pregnancy loss and -863C/A (p=0.000), -308G/A (p=0.045), and -238G/A (p=0.034) polymorphisms. TNF-? polymorphisms of -863C and -238G may be susceptible factors of recurrent pregnancy loss cases. The -308G polymorphism has an important role in maintaining pregnancy. Conclusion:The -863C/A and -238G/A TNF-? polymorphisms are possible genetic risk factors of recurrent pregnancy loss and might be its predictive markers.

SUBMITTER: Aboutorabi R

PROVIDER: S-EPMC6328976 | biostudies-literature | 2018 Oct-Dec

REPOSITORIES: biostudies-literature

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The Study of Association Between Polymorphism of TNF-α Gene's Promoter Region and Recurrent Pregnancy Loss.

Aboutorabi Roshanak R Behzadi Ehsan E Sadegh Mohammad Javad MJ Fatehi Seyed Pooriya SP Semsarzadeh Soroosh S Zarrin Yasaman Y Kazemi Mohammad M Rafiee Laleh L Mostafavi Fatemeh Sadat FS

Journal of reproduction & infertility 20181001 4

<h4>Background</h4>According to the literature review, polymorphisms of tumor necrosis factor alpha's (TNF-α) promoter region are probably the genetic risk factors of recurrent pregnancy loss. This study has investigated five single nucleotide polymorphisms in the TNF-α gene's promoter region to evaluate their relationship with recurrent pregnancy loss disorder.<h4>Methods</h4>Blood samples were taken from 65 women with recurrent pregnancy loss (Case group) and 65 healthy women with a history of ...[more]

PMID: 30746336

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Project description:Background:Multiple studies have been carried out examining the association of tumor necrosis factor-? gene (TNF-?) promoter region polymorphisms with recurrent pregnancy loss (RPL) risk. However, the results remain controversial and incomplete. Hence, we performed a meta-analysis to evaluate the association of the TNF-? -308G>A and -238G>A polymorphisms with RPL risk. Materials and Methods:In this meta-analysis, a comprehensive search of PubMed, Web of Knowledge and EMBASE was performed to identify relevant studies published until December 1, 2017. The associations were assessed by odds ratio (OR) and its corresponding 95% confidence interval (CI). Results:A total of 29 case-control studies, comprising 20 studies on TNF-? -308G>A (3,461 cases and 3,895 controls) and nine studies on TNF-? -238G>A (2,589 cases and 2,664 controls), were included in the meta-analysis. Overall, we found TNF-? -308G>A to be associated with an increase in RPL risk under the homozygote (OR=1.716, 95% CI: 1.210-2.433, P=0.002) and the recessive (OR=1.554, 95% CI: 1.100-2.196, P=0.012) models. TNF-? -238G>A was also significantly associated with increased risk of RPL under the allele model (OR=1.554, 95% CI: 1.100-2.196, P=0.012). Stratified analysis revealed a more significant association between theTNF-? -308G>A polymorphism and increased RPL risk in Asians under the homozygote (OR=2.190, 95% CI: 1.465-3.274, P?0.001), the dominant (OR=1.642, 95% CI: 1.269-2.125, P?0.001) and the recessive (OR=1.456, 95% CI: 1.039-2.040, P=0.029) models, but not in Caucasians. A non-significant association was, however, identified between TNF-? -238G>A and RPL risk based on ethnicity. Moreover, TNF-? -308G>A and -238G>A polymorphisms were significantly associated with increased risk of RPL in high quality studies and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) subgroups. Conclusion:The present meta-analysis demonstrates that TNF-? -308G>A and -238G>A polymorphisms are associated with an increased risk of RPL.

Dataset Information

The Study of Association Between Polymorphism of TNF-? Gene's Promoter Region and Recurrent Pregnancy Loss.

Publications

The Study of Association Between Polymorphism of TNF-α Gene's Promoter Region and Recurrent Pregnancy Loss.

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