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Guidelines for DNA recombination and repair studies: Cellular assays of DNA repair pathways.


ABSTRACT: Understanding the plasticity of genomes has been greatly aided by assays for recombination, repair and mutagenesis. These assays have been developed in microbial systems that provide the advantages of genetic and molecular reporters that can readily be manipulated. Cellular assays comprise genetic, molecular, and cytological reporters. The assays are powerful tools but each comes with its particular advantages and limitations. Here the most commonly used assays are reviewed, discussed, and presented as the guidelines for future studies.

SUBMITTER: Klein HL 

PROVIDER: S-EPMC6334234 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature

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Guidelines for DNA recombination and repair studies: Cellular assays of DNA repair pathways.

Klein Hannah L HL   Bačinskaja Giedrė G   Che Jun J   Cheblal Anais A   Elango Rajula R   Epshtein Anastasiya A   Fitzgerald Devon M DM   Gómez-González Belén B   Khan Sharik R SR   Kumar Sandeep S   Leland Bryan A BA   Marie Léa L   Mei Qian Q   Miné-Hattab Judith J   Piotrowska Alicja A   Polleys Erica J EJ   Putnam Christopher D CD   Radchenko Elina A EA   Saada Anissia Ait AA   Sakofsky Cynthia J CJ   Shim Eun Yong EY   Stracy Mathew M   Xia Jun J   Yan Zhenxin Z   Yin Yi Y   Aguilera Andrés A   Argueso Juan Lucas JL   Freudenreich Catherine H CH   Gasser Susan M SM   Gordenin Dmitry A DA   Haber James E JE   Ira Grzegorz G   Jinks-Robertson Sue S   King Megan C MC   Kolodner Richard D RD   Kuzminov Andrei A   Lambert Sarah Ae SA   Lee Sang Eun SE   Miller Kyle M KM   Mirkin Sergei M SM   Petes Thomas D TD   Rosenberg Susan M SM   Rothstein Rodney R   Symington Lorraine S LS   Zawadzki Pawel P   Kim Nayun N   Lisby Michael M   Malkova Anna A  

Microbial cell (Graz, Austria) 20190107 1


Understanding the plasticity of genomes has been greatly aided by assays for recombination, repair and mutagenesis. These assays have been developed in microbial systems that provide the advantages of genetic and molecular reporters that can readily be manipulated. Cellular assays comprise genetic, molecular, and cytological reporters. The assays are powerful tools but each comes with its particular advantages and limitations. Here the most commonly used assays are reviewed, discussed, and prese  ...[more]

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