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Chitosan-assisted permeabilization of ATP-biotin for live cell kinase-catalyzed biotinylation.

ABSTRACT: Kinases are essential cell signaling enzymes that phosphorylate protein substrates using ATP as the universal cosubstrate. A wide variety of ATP analogs have been used in kinase research, although the studies are limited by the cell impermeability of ATP. Here we describe the use of the cationic polymer deacetylated chitosan to permeabilize ATP analogs for live cell applications, including kinase-catalyzed biotinylation.

SUBMITTER: Fouda AE

PROVIDER: S-EPMC6335084 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature

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Chitosan-assisted permeabilization of ATP-biotin for live cell kinase-catalyzed biotinylation.

Fouda Ahmed E AE Embogama D Maheeka DM Ramanayake-Mudiyanselage Vindya V H Pflum Mary Kay MK

BioTechniques 20180901 3

Kinases are essential cell signaling enzymes that phosphorylate protein substrates using ATP as the universal cosubstrate. A wide variety of ATP analogs have been used in kinase research, although the studies are limited by the cell impermeability of ATP. Here we describe the use of the cationic polymer deacetylated chitosan to permeabilize ATP analogs for live cell applications, including kinase-catalyzed biotinylation. ...[more]

PMID: 30227738

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