Ontology highlight
ABSTRACT:
SUBMITTER: Fouda AE
PROVIDER: S-EPMC4551444 | biostudies-literature | 2015 Aug
REPOSITORIES: biostudies-literature
Fouda Ahmed E AE Pflum Mary Kay H MK
Angewandte Chemie (International ed. in English) 20150626 33
ATP analogues have been powerful compounds for the study of kinase-catalyzed phosphorylation. However, the cell impermeability of ATP analogues has largely limited their use to in vitro lysate-based experiments. Herein, we report the first cell-permeable ATP analogue, ATP-polyamine-biotin (APB). APB is shown to promote biotin labeling of kinase substrates in live cells and has future applications in phosphoprotein purification and analysis. More generally, these studies provide a foundation for ...[more]