Ontology highlight
ABSTRACT:
SUBMITTER: Wang X
PROVIDER: S-EPMC6339868 | biostudies-literature | 2018 Jul
REPOSITORIES: biostudies-literature
Wang Xiao X Allen William E WE Wright Matthew A MA Sylwestrak Emily L EL Samusik Nikolay N Vesuna Sam S Evans Kathryn K Liu Cindy C Ramakrishnan Charu C Liu Jia J Nolan Garry P GP Bava Felice-Alessio FA Deisseroth Karl K
Science (New York, N.Y.) 20180621 6400
Retrieving high-content gene-expression information while retaining three-dimensional (3D) positional anatomy at cellular resolution has been difficult, limiting integrative understanding of structure and function in complex biological tissues. We developed and applied a technology for 3D intact-tissue RNA sequencing, termed STARmap (spatially-resolved transcript amplicon readout mapping), which integrates hydrogel-tissue chemistry, targeted signal amplification, and in situ sequencing. The capa ...[more]