Unknown

Dataset Information

0

Cell-free formation of RNA granules: low complexity sequence domains form dynamic fibers within hydrogels.


ABSTRACT: Eukaryotic cells contain assemblies of RNAs and proteins termed RNA granules. Many proteins within these bodies contain KH or RRM RNA-binding domains as well as low complexity (LC) sequences of unknown function. We discovered that exposure of cell or tissue lysates to a biotinylated isoxazole (b-isox) chemical precipitated hundreds of RNA-binding proteins with significant overlap to the constituents of RNA granules. The LC sequences within these proteins are both necessary and sufficient for b-isox-mediated aggregation, and these domains can undergo a concentration-dependent phase transition to a hydrogel-like state in the absence of the chemical. X-ray diffraction and EM studies revealed the hydrogels to be composed of uniformly polymerized amyloid-like fibers. Unlike pathogenic fibers, the LC sequence-based polymers described here are dynamic and accommodate heterotypic polymerization. These observations offer a framework for understanding the function of LC sequences as well as an organizing principle for cellular structures that are not membrane bound.

SUBMITTER: Kato M 

PROVIDER: S-EPMC6347373 | biostudies-literature | 2012 May

REPOSITORIES: biostudies-literature

altmetric image

Publications


Eukaryotic cells contain assemblies of RNAs and proteins termed RNA granules. Many proteins within these bodies contain KH or RRM RNA-binding domains as well as low complexity (LC) sequences of unknown function. We discovered that exposure of cell or tissue lysates to a biotinylated isoxazole (b-isox) chemical precipitated hundreds of RNA-binding proteins with significant overlap to the constituents of RNA granules. The LC sequences within these proteins are both necessary and sufficient for b-i  ...[more]

Similar Datasets

| S-EPMC6087745 | biostudies-literature
| S-EPMC4183792 | biostudies-literature
| S-EPMC4534042 | biostudies-literature
| S-EPMC6093899 | biostudies-literature
| S-EPMC7399500 | biostudies-literature
| S-EPMC7704730 | biostudies-literature
| S-EPMC7555853 | biostudies-literature
| S-EPMC4076346 | biostudies-literature
| S-EPMC3812964 | biostudies-literature
| S-EPMC6643637 | biostudies-literature