Ontology highlight
ABSTRACT:
SUBMITTER: Kato M
PROVIDER: S-EPMC6347373 | biostudies-literature | 2012 May
REPOSITORIES: biostudies-literature
Kato Masato M Han Tina W TW Xie Shanhai S Shi Kevin K Du Xinlin X Wu Leeju C LC Mirzaei Hamid H Goldsmith Elizabeth J EJ Longgood Jamie J Pei Jimin J Grishin Nick V NV Frantz Douglas E DE Schneider Jay W JW Chen She S Li Lin L Sawaya Michael R MR Eisenberg David D Tycko Robert R McKnight Steven L SL
Cell 20120501 4
Eukaryotic cells contain assemblies of RNAs and proteins termed RNA granules. Many proteins within these bodies contain KH or RRM RNA-binding domains as well as low complexity (LC) sequences of unknown function. We discovered that exposure of cell or tissue lysates to a biotinylated isoxazole (b-isox) chemical precipitated hundreds of RNA-binding proteins with significant overlap to the constituents of RNA granules. The LC sequences within these proteins are both necessary and sufficient for b-i ...[more]