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A Novel Anti-EGFR mAb Ame55 with Lower Toxicity and Better Efficacy than Cetuximab When Combined with Irinotecan.

ABSTRACT: To improve efficacy and minimize toxicity of EGFR inhibition treatment, we developed Ame55, a novel anti-EGFR IgG1 with lower affinity to EGFR than cetuximab (C225) from a human phage library. Ame55 had lower bioactivity than cetuximab in vitro but similar antitumor efficacy as cetuximab in vivo. Moreover, Ame55 was more efficacious than cetuximab in a Lovo cell xenograft tumor model when combined with irinotecan (CPT-11). Ame55 concentrates in the mouse xenograft tumor and has less toxicity than cetuximab in cynomolgus monkeys in an overdose study.

SUBMITTER: Qiu W

PROVIDER: S-EPMC6348820 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature

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A Novel Anti-EGFR mAb Ame55 with Lower Toxicity and Better Efficacy than Cetuximab When Combined with Irinotecan.

Qiu Weiyi W Zhang Chang C Wang Shuang S Yu Xiaoyan X Wang Qiong Q Zeng Dadi D Du Peng P Ma Jinling J Zheng Yiqiong Y Pang Bo B Yu Yunzhou Y Long Feng F Pang Xiaobin X Sun Zhiwei Z

Journal of immunology research 20190113

To improve efficacy and minimize toxicity of EGFR inhibition treatment, we developed Ame55, a novel anti-EGFR IgG1 with lower affinity to EGFR than cetuximab (C225) from a human phage library. Ame55 had lower bioactivity than cetuximab <i>in vitro</i> but similar antitumor efficacy as cetuximab <i>in vivo</i>. Moreover, Ame55 was more efficacious than cetuximab in a Lovo cell xenograft tumor model when combined with irinotecan (CPT-11). Ame55 concentrates in the mouse xenograft tumor and has les ...[more]

PMID: 30733972

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