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Microfluidic synthesis and on-chip enrichment application of two-dimensional hollow sandwich-like mesoporous silica nanosheet with water ripple-like surface.

ABSTRACT: The merits of microfluidics bring new opportunities for engineering of nanomaterials with well controlled chemical, physical and biological properties for a variety of applications. Herein, using a two-run spiral-shaped microfluidic device, we first develop a facile and straightforward flow synthesis strategy to create two-dimensional mesoporous silica nanosheet (MSN). Such MSN exhibits typical hollow sandwich-like bilayer and unique water ripple-like wrinkle surface, which greatly increase the particulate accessibility for mass transfer. The enhanced on-chip enrichment performance of MSN is further confirmed toward different substrates (dye, protein, drug). These findings not only shed new light on microfluidics-enabled chemicals engineering, but also open up numerous new possibilities by microfluidics in adsorption, separation, and biomedical fields.

SUBMITTER: Hao N

PROVIDER: S-EPMC6351192 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature

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Microfluidic synthesis and on-chip enrichment application of two-dimensional hollow sandwich-like mesoporous silica nanosheet with water ripple-like surface.

Hao Nanjing N Nie Yuan Y Closson Andrew B AB Zhang John X J JXJ

Journal of colloid and interface science 20181211

The merits of microfluidics bring new opportunities for engineering of nanomaterials with well controlled chemical, physical and biological properties for a variety of applications. Herein, using a two-run spiral-shaped microfluidic device, we first develop a facile and straightforward flow synthesis strategy to create two-dimensional mesoporous silica nanosheet (MSN). Such MSN exhibits typical hollow sandwich-like bilayer and unique water ripple-like wrinkle surface, which greatly increase the ...[more]

PMID: 30576991

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Project description:Tunable glutathione (GSH)-sensitive hollow mesoporous silica nanoparticles (HMSiO2 NPs) were developed using a structural difference-based selective etching strategy. These organosilica hollow nanoparticles contained disulfide linkages (S-S) in the outer shell which were degraded by GSH. The particles were compared with their nonGSH-sensitive tetraethyl orthosilicate (TEOS) HMSiO2 counterparts in terms of their synthesis method, characterization, doxorubicin (DOX) release profile, and in vitro cytotoxicity in MCF-7 breast cancer cells. Transmission electron microscopy (TEM) of the particles indicated that the fabricated HMSiO2 NPs had an average diameter of 130?±?5?nm. Thermogravimetric analysis (TGA) revealed that GSH-sensitive particles had approximately 5.3% more weight loss than TEOS HMSiO2 NPs. Zeta potential of these redox-responsive particles was -23?±?1?mV at pH?6 in deionized (DI) water. Nitrogen adsorption-desorption isotherm revealed that the surface area of the hollow mesoporous nanoreservoirs was roughly 446?±?6?m2?g-1 and the average diameter of the pores was 2.3?±?0.5?nm. TEM images suggest that the nanoparticles started to lose mass integrity from Day 1. The particles showed a high loading capacity for DOX (8.9?±?0.5%) as a model drug, due to the large voids existing in the hollow structures. Approximately 58% of the incorporated DOX released within 14?days in phosphate buffered saline (PBS) at pH?6 and in the presence of 10?mM of GSH, mimicking intracellular tumor microenvironment while release from TEOS HMSiO2 NPs was only c.a. 18%. The uptake of these hollow nanospheres by MCF-7 cells and RAW 264.7 macrophages was evaluated using TEM and confocal microscopy. The nanospheres were shown to accumulate in the endolysosomal compartments after incubation for 24?h with the maximum uptake of c.a. 2.1?±?0.3% and 5.2?±?0.4%, respectively. Cytotoxicity of the nanospheres was investigated using CCK-8 assay. Results indicate that intact hollow particles (both GSH-sensitive and TEOS HMSiO2 NPs) were nontoxic to MCF-7 cells after incubation for 24?h within the concentration range of 0-1000??g?ml-1. DOX-loaded GSH-sensitive nanospheres containing 6??g?ml-1 of DOX killed c.a. 51% of MCF-7 cells after 24?h while TEOS HMSiO2 NPs killed c.a. 20% with the difference being statistically significant. Finally, cytotoxicity data in RAW 264.7 macrophages and NIH 3?T3 fibroblasts shows that intact GSH-sensitive HMSiO2 NPs did not show any toxic effects on these cells with the concentrations equal or <125??g?ml-1.

Dataset Information

Microfluidic synthesis and on-chip enrichment application of two-dimensional hollow sandwich-like mesoporous silica nanosheet with water ripple-like surface.

Publications

Microfluidic synthesis and on-chip enrichment application of two-dimensional hollow sandwich-like mesoporous silica nanosheet with water ripple-like surface.

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