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Complexes of Pro-Apoptotic siRNAs and Carbosilane Dendrimers: Formation and Effect on Cancer Cells.

ABSTRACT: This paper examines the complexation of anti-cancer small interfering RNAs (siRNAs) by cationic carbosilane dendrimers, and the interaction of the formed complexes with HeLa and HL-60 cancer cells. Stepwise formation of the complexes accompanied by the evolution of their properties has been observed through the increase of the charge ratio (dendrimer/siRNA). The complexes decrease the viability of both "easy-to-transfect" cells (HeLa) and "hard-to transfect" ones (HL-60), indicating a high potential of the cationic carbosilane dendrimers for siRNA delivery into tumor cells.

SUBMITTER: Krasheninina OA

PROVIDER: S-EPMC6359069 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature

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Complexes of Pro-Apoptotic siRNAs and Carbosilane Dendrimers: Formation and Effect on Cancer Cells.

Krasheninina Olga A OA Apartsin Evgeny K EK Fuentes Elena E Szulc Aleksandra A Ionov Maksim M Venyaminova Alya G AG Shcharbin Dzmitry D de la Mata F Javier FJ Bryszewska Maria M Gόmez Rafael R

Pharmaceutics 20190110 1

This paper examines the complexation of anti-cancer small interfering RNAs (siRNAs) by cationic carbosilane dendrimers, and the interaction of the formed complexes with HeLa and HL-60 cancer cells. Stepwise formation of the complexes accompanied by the evolution of their properties has been observed through the increase of the charge ratio (dendrimer/siRNA). The complexes decrease the viability of both "easy-to-transfect" cells (HeLa) and "hard-to transfect" ones (HL-60), indicating a high poten ...[more]

PMID: 30634643

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