Dataset Information

Sodium Selenate Treatment Using a Combination of Seed Priming and Foliar Spray Alleviates Salinity Stress in Rice.

ABSTRACT: Soil salinity is one of the important abiotic stress factors that affect rice productivity and quality. Research with several dicotyledonous plants indicated that the detrimental effects associated with salinity stress can (partly) be overcome by the external application of antioxidative substances. For instance, sodium selenate (Na₂SeO₄) significantly improved the growth and productivity of several crops under various abiotic stress conditions. At present there is no report describing the impact of Na₂SeO₄ on salinity stressed cereals such as rice. Rice cultivation is threatened by increasing salinity stress, and in future this problem will further be aggravated by global warming and sea level rise, impacting coastal areas. The current study reports on the effect of Na₂SeO₄ in alleviating salinity stress in rice plants. The optimal concentration of Na₂SeO₄ and the most efficient mode of selenium application were investigated. Selenium, sodium, and potassium contents in leaves were determined. Antioxidant enzyme activities as well as proline, hydrogen peroxide (H₂O₂), and malondialdehyde (MDA) concentrations were analyzed. In addition, the transcript levels for OsNHX1, an important Na⁺/H⁺ antiporter, were quantified. Treatment of 2-week-old rice plants under 150 mM NaCl stress with 6 mg l^-1 Na₂SeO₄ improved the total biomass. A significantly higher biomass was observed for the plants that received Na₂SeO₄ by a combination of seed priming and foliar spray compared to the individual treatments. The Na₂SeO₄ application enhanced the activity of antioxidant enzymes (SOD, APX, CAT, and GSH-Px), increased the proline content, and reduced H₂O₂ and MDA concentrations in plants under NaCl stress. These biochemical changes were accompanied by increased transcript levels for OsNHX1 resulting in a higher K⁺/Na⁺ ratio in the rice plants under NaCl stress. The results suggest that Na₂SeO₄ treatment alleviates the adverse effect of salinity on rice plant growth through enhancing the antioxidant defense system and increase of OsNHX1 transcript levels.

SUBMITTER: Subramanyam K

PROVIDER: S-EPMC6378292 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature

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Sodium Selenate Treatment Using a Combination of Seed Priming and Foliar Spray Alleviates Salinity Stress in Rice.

Subramanyam Kondeti K Du Laing Gijs G Van Damme Els J M EJM

Frontiers in plant science 20190211

Soil salinity is one of the important abiotic stress factors that affect rice productivity and quality. Research with several dicotyledonous plants indicated that the detrimental effects associated with salinity stress can (partly) be overcome by the external application of antioxidative substances. For instance, sodium selenate (Na<sub>2</sub>SeO<sub>4</sub>) significantly improved the growth and productivity of several crops under various abiotic stress conditions. At present there is no repor ...[more]

PMID: 30804974

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Project description:BACKGROUND:Selenium (Se) is a beneficial element for higher plants and essential for mammals. To study the effect of the foliar application of sodium selenate on fragrant rice performance, a pot experiment was conducted in Guangdong, China. At the initial heading stage, one-time foliar application of sodium selenate with concentrations of 0, 10, 20, 30, 40 and 50??mol·L-?1 (named CK, Se1, Se2, Se3, Se4 and Se5, respectively) were foliar applied on two fragrant rice varieties, 'Meixiangzhan-2' and 'Xiangyaxiangzhan'. RESULTS:Selenate application at the initial heading stage not only improved the grain yield of fragrant rice by increasing the seed-setting rate and grain weight, but also promoted the grain quality by increasing crude protein contents and lowering the chalky rice rate. Furthermore, Se applications enhanced the biosynthesis of 2-acetyl-1- pyrroline (2-AP), the main aromatic compound, by increasing the contents of precursors (?1- pyrroline, proline and pyrroline-5-carboxylic acid (P5C)) and the activities of enzymes (proline dehydrogenase (PRODH), ?1-pyrroline-5-carboxylic acid synthetase (P5CS), and ornithine aminotransferase (OAT)) in fragrant rice. The results also showed that foliar application of sodium selenate enhanced the antioxidant system of both varieties by promoting the activities of peroxidase (POD), superoxide dismutase (SOD), catalase (CAT) and reducing the contents of malondialdehyde (MDA). Furthermore, the real-time PCR analyses depicted that foliar application of selenate up-regulated the GPX1, GPX4 and CATC transcripts. The higher antioxidative enzymatic activities might strength the stress resistant to ensure the stability of yield in fragrant rice form abiotic stress. CONCLUSIONS:Foliar applications of sodium selenate at the initial heading stage increased the grain 2-AP content by enhancing the biosynthesis-related enzymes and precursors. The grain yield and quality of fragrant rice also increased due to selenate application. Furthermore, foliar application of selenate promoted the activities of enzymes such as POD, SOD and CAT and up-regulated the expression of gene GPX4, GPX1 and CATC.

Dataset Information

Sodium Selenate Treatment Using a Combination of Seed Priming and Foliar Spray Alleviates Salinity Stress in Rice.

Publications

Sodium Selenate Treatment Using a Combination of Seed Priming and Foliar Spray Alleviates Salinity Stress in Rice.

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