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Pectin and Neutral Monosaccharides Production during the Simultaneous Hydrothermal Extraction of Waste Biomass from Refining of Sugar-Optimization with the Use of Doehlert Design.

ABSTRACT: We propose a one-stage hydrothermal extraction of sugar beet pulp leading to effective co-production of pectin and neutral monosaccharides with a relatively high yield and satisfactory purity without the presence of an acidic catalyst. The optimal experimental design methodology was used for modelling and optimizing the yield of pectin and neutral monosaccharides. In good agreement with experimental results (R² = 0.955), the model predicts an optimal yield of pectin (approx. 121.1 g kg-1 ± 0.47 g kg-1) at a temperature and time of about 118.1 °C and 21.5 min, respectively. The highest yield of the sum of neutral monosaccharides (approx. 82.6 g kg-1 ± 0.72 g kg-1) was obtained at about 116.2 °C and 26.4 min (R² = 0.976). The obtained results are suitable for industrial upscaling and may provide an incentive to implement a new, environmentally friendly, simple, and effective method for treating waste product from the sugar refining industry, which has proved onerous until now.

SUBMITTER: Pinkowska H

PROVIDER: S-EPMC6385035 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature

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Pectin and Neutral Monosaccharides Production during the Simultaneous Hydrothermal Extraction of Waste Biomass from Refining of Sugar-Optimization with the Use of Doehlert Design.

Pińkowska Hanna H Krzywonos Małgorzata M Wolak Paweł P Złocińska Adrianna A

Molecules (Basel, Switzerland) 20190129 3

We propose a one-stage hydrothermal extraction of sugar beet pulp leading to effective co-production of pectin and neutral monosaccharides with a relatively high yield and satisfactory purity without the presence of an acidic catalyst. The optimal experimental design methodology was used for modelling and optimizing the yield of pectin and neutral monosaccharides. In good agreement with experimental results (R² = 0.955), the model predicts an optimal yield of pectin (approx. 121.1 g kg<sup>-1</s ...[more]

PMID: 30699933

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Project description:Dietary fiber, with intake of soluble fibers in particular, has been reported to lower the risk for developing inflammatory bowel diseases (IBD). This is at least partly attributable to the fermentation of dietary fiber by the colonic microbiota to produce short chain fatty acids. Pectin, a widely consumed soluble fiber, is known to exert a protective effect in murine models of IBD, but the underlying mechanism remains elusive. Apart from having a prebiotic effect, it has been suggested that pectin direct influences host cells by modulating the inflammatory response in a manner dependent on its neutral sugar side chains. Here we examined the effect of the side chain content of pectin on the pathogenesis of experimental colitis in mice. Male C57BL/6 mice were fed a pectin-free diet, or a diet supplemented with characteristically high (5% orange pectin) or low (5% citrus pectin) side chain content for 10-14 days, and then administered 2,4,6-trinitrobenzene sulfonic acid or dextran sulfate sodium to induce colitis. We found that the clinical symptoms and tissue damage in the colon were ameliorated in mice that were pre-fed with orange pectin, but not in those pre-fed with citrus pectin. Although the population of CD4+Foxp+ regulatory T cells and CD4+RORγt+ inflammatory T cells in the colon were comparable between citrus and orange pectin-fed mice, colonic interleukin (IL)-1β and IL-6 levels in orange pectin-fed mice were significantly decreased. The fecal concentration of propionic acid in orange pectin-fed mice was slightly but significantly higher than that in control and citrus pectin-fed mice but the cecal concentration of propionic acid after the induction of TNBS colitis was comparable between orange and citrus pectin-fed mice. Furthermore, the protective effect of orange pectin against colitis was observed even in mice treated with antibiotics. IL-6 production from RAW264.7 cells stimulated with the toll-like receptor agonist Pam3CSK4 or lipopolysaccharide was suppressed by pre-treatment with orange pectin in vitro. Taken together, these results suggest that the side chains of pectin not only augment prebiotic effects but also directly regulate IL-6 production from intestinal host cells in a microbiota-independent fashion to attenuate colitis.

Project description:Aucubin (AU) is an active ingredient exerting strong antioxidant and anti-inflammatory effects in treating several diseases. This study evaluated the extraction of AU from Eucommia ulmoides seed-draff (EUSD) waste biomass using a series of solvents (methanol, ethanol, i-propanol, n-propanol, n-butanol, n-pentanol and cyclohexane) assisted with microwave and ultrasound, and proposed the optimized method for extraction. Five factors were investigated by Box-Behnken design (BBD) and response surface methodology (RSM). The optimized extraction conditions were as follows: liquid-solid ratio of 46.37 mL/g, methanol percentage of 89.56%, ultrasonic (extraction) time of 59.95 min, microwave power of 306.73 W, and microwave (extraction) time of 18.93 s. To this end, the AU extraction reached the maximum value (149.1 mg/g), which was consistent with the theoretical value (149.3 mg/g). Furthermore, the kinetics of extraction process were investigated by mathematic modeling. The extraction process analysis was also explored by 1H nuclear magnetic resonance (1H-NMR) spectroscopy, Fourier transform infrared (FTIR) spectroscopy and COSMOtherm program. This study found out that methanol provided better extraction efficiency than the conventional solvents (water, ethanol, i-propanol, n-propanol, n-butanol, n-pentanol, cyclohexane) due to possible interactions by the formation of hydrogen bond between AU and methanol, and ultrasound and microwave could significantly enhance mass transfer, which exhibited higher extraction efficiency and lower energy consumptions (149.1 mg/g and 0.102 kW·h vs. 73.4 mg/g and 0.700 kW·h for Soxhlet extraction). In the antibacterial activity study, the AU extract exerted strong antibacterial ability against 4 tested pathogens, and the antibacterial effect followed the order of: Staphylococcus aureus (35.9 ± 1.32 mm) > Escherichia coli (30.7 ± 1.38 mm) > Bacillus subtilis (20.5 ± 1.36 mm) > Salmonella (15.9 ± 1.39 mm) with the AU concentration of 40 mg/mL. Therefore, the development of this study will help to deepen the further understanding of natural product extraction by methanol-based ultrasonic and microwave, and has certain application value for the development and utilization of natural iridoid glycosides product.

Dataset Information

Pectin and Neutral Monosaccharides Production during the Simultaneous Hydrothermal Extraction of Waste Biomass from Refining of Sugar-Optimization with the Use of Doehlert Design.

Publications

Pectin and Neutral Monosaccharides Production during the Simultaneous Hydrothermal Extraction of Waste Biomass from Refining of Sugar-Optimization with the Use of Doehlert Design.

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