Correction to: Comparison of whole-genome bisulfite sequencing library preparation strategies identifies sources of biases affecting DNA methylation data.
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ABSTRACT:
SUBMITTER: Olova N
PROVIDER: S-EPMC6385436 | biostudies-literature | 2019 Feb
REPOSITORIES: biostudies-literature
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Correction to: Comparison of whole-genome bisulfite sequencing library preparation strategies identifies sources of biases affecting DNA methylation data.
Olova Nelly N Krueger Felix F Andrews Simon S Oxley David D Berrens Rebecca V RV Branco Miguel R MR Reik Wolf W
Genome biology 20190222 1
Following publication of the original article [1], it was reported that the incorrect "Additional file 3" was published. The correct additional file is given below. ...[more]
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Project description:We performed a parallel analysis of commonly used pre- and post-bisulfite WGBS library preparation protocols for their performance and quality of sequencing outputs. Our results show that bisulfite conversion per se generates pronounced sequencing biases, and subsequent fragmentation and amplification steps lead to several-fold overrepresentation of these artefacts. Standard pre-bisulfite library preparation methods lead to a significantly biased genomic sequence representation and a marked overestimation of methylation levels. We have integrated a bias diagnostic tool in the Bismark package and propose that amplification-free and post-bisulfite procedures should become the gold standard for WGBS library preparation.
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