Ontology highlight
ABSTRACT:
SUBMITTER: O'Brien AR
PROVIDER: S-EPMC6391469 | biostudies-literature | 2019 Feb
REPOSITORIES: biostudies-literature
O'Brien Aidan R AR Wilson Laurence O W LOW Burgio Gaetan G Bauer Denis C DC Bauer Denis C DC
Scientific reports 20190226 1
Editing individual nucleotides is a crucial component for validating genomic disease association. It is currently hampered by CRISPR-Cas-mediated "base editing" being limited to certain nucleotide changes, and only achievable within a small window around CRISPR-Cas target sites. The more versatile alternative, HDR (homology directed repair), has a 3-fold lower efficiency with known optimization factors being largely immutable in experiments. Here, we investigated the variable efficiency-governin ...[more]