Ontology highlight
ABSTRACT:
SUBMITTER: Jarrell JA
PROVIDER: S-EPMC6397276 | biostudies-literature | 2019 Mar
REPOSITORIES: biostudies-literature
Jarrell Justin A JA Twite Amy A AA Lau Katherine H W J KHWJ Kashani Moein N MN Lievano Adrian A AA Acevedo Julyana J Priest Craig C Nieva Jorge J Gottlieb David D Pawell Ryan S RS
Scientific reports 20190301 1
Intracellular delivery of functional macromolecules, such as DNA and RNA, across the cell membrane and into the cytosol, is a critical process in both biology and medicine. Herein, we develop and use microfluidic chips containing post arrays to induce microfluidic vortex shedding, or μVS, for cell membrane poration that permits delivery of mRNA into primary human T lymphocytes. We demonstrate transfection with μVS by delivery of a 996-nucleotide mRNA construct encoding enhanced green fluorescent ...[more]