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Hyperbaric oxygen improves functional recovery of rats after spinal cord injury via activating stromal cell-derived factor-1/CXC chemokine receptor 4 axis and promoting brain-derived neurothrophic factor expression.

ABSTRACT: BACKGROUND:Spinal cord injury (SCI) is a worldwide medical concern. This study aimed to elucidate the mechanism underlying the protective effect of hyperbaric oxygen (HBO) against SCI-induced neurologic defects in rats via exploring the stromal cell-derived factor-1 (SDF-1)/CXC chemokine receptor 4 (CXCR4) axis and expression of brain-derived neurotrophic factor (BDNF). METHODS:An acute SCI rat model was established in Sprague-Dawley rats using the Allen method. Sixty rats were divided into four groups (n?=?15 in each group): sham-operated, SCI, SCI treated with HBO (SCI?+?HBO), and SCI treated with both HBO and AMD3100 (an antagonist of CXCR4; SCI?+?HBO?+?AMD) groups. The rats were treated with HBO twice a day for 3 days and thereafter once a day after the surgery for up to 28 days. Following the surgery, neurologic assessments were performed with the Basso-Bettie-Bresnahan (BBB) scoring system on postoperative day (POD) 7, 14, 21, and 28. Spinal cord tissues were harvested to assess the expression of SDF-1, CXCR4, and BDNF at mRNA and protein levels, using quantitative real-time polymerase chain reaction, Western blot analysis, and histopathologic analysis. RESULTS:HBO treatment recovered SCI-induced descent of BBB scores on POD 14, (1.25?±?0.75 vs. 1.03?±?0.66, P?

SUBMITTER: Meng XL

PROVIDER: S-EPMC6416102 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature

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The CXC chemokine receptor 4 ligands ubiquitin and stromal cell-derived factor-1? function through distinct receptor interactions.

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