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Complete nucleotide sequence of a novel mycovirus from Trichoderma harzianum in China.

ABSTRACT: A new mycovirus was identified in Trichoderma harzianum strain 137 isolated in Xinjiang province, China. The whole genome sequence of the mycovirus was determined by metagenomic sequencing, RT-PCR, and RACE cloning. The mycovirus contained two genomic segments. The first segment was 2088 bp long and contained a single ORF (ORF1) encoding the RNA-dependent RNA polymerase (RdRP) (72.26 kDa). The second segment was 1634 bp long and also contained a single ORF (ORF2) encoding a hypothetical protein of 37.472 kDa. We named this novel mycovirus "Trichoderma harzianum bipartite mycovirus 1" (ThBMV1). Phylogenetic analysis showed that ThBMV1 clusters with other unclassified dsRNA mycoviruses.

SUBMITTER: Liu C

PROVIDER: S-EPMC6420475 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature

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Complete nucleotide sequence of a novel mycovirus from Trichoderma harzianum in China.

Liu Chenchen C Li Mei M Redda Estifanos Tsegaye ET Mei Jie J Zhang Jiantai J Elena Santiago F SF Wu Beilei B Jiang Xiliang X

Archives of virology 20190212 4

A new mycovirus was identified in Trichoderma harzianum strain 137 isolated in Xinjiang province, China. The whole genome sequence of the mycovirus was determined by metagenomic sequencing, RT-PCR, and RACE cloning. The mycovirus contained two genomic segments. The first segment was 2088 bp long and contained a single ORF (ORF1) encoding the RNA-dependent RNA polymerase (RdRP) (72.26 kDa). The second segment was 1634 bp long and also contained a single ORF (ORF2) encoding a hypothetical protein ...[more]

PMID: 30746561

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