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Low-Cost Cellulase-Hemicellulase Mixture Secreted by Trichoderma harzianum EM0925 with Complete Saccharification Efficacy of Lignocellulose.


ABSTRACT: Fermentable sugars are important intermediate products in the conversion of lignocellulosic biomass to biofuels and other value-added bio-products. The main bottlenecks limiting the production of fermentable sugars from lignocellulosic biomass are the high cost and the low saccharification efficiency of degradation enzymes. Herein, we report the secretome of Trichoderma harzianum EM0925 under induction of lignocellulose. Numerously and quantitatively balanced cellulases and hemicellulases, especially high levels of glycosidases, could be secreted by T. harzianum EM0925. Compared with the commercial enzyme preparations, the T. harzianum EM0925 enzyme cocktail presented significantly higher lignocellulolytic enzyme activities and hydrolysis efficiency against lignocellulosic biomass. Moreover, 100% yields of glucose and xylose were obtained simultaneously from ultrafine grinding and alkali pretreated corn stover. These findings demonstrate a natural cellulases and hemicellulases mixture for complete conversion of biomass polysaccharide, suggesting T. harzianum EM0925 enzymes have great potential for industrial applications.

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC7014229 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature

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Low-Cost Cellulase-Hemicellulase Mixture Secreted by <i>Trichoderma harzianum</i> EM0925 with Complete Saccharification Efficacy of Lignocellulose.

Zhang Yu Y   Yang Jinshui J   Luo Lijin L   Wang Entao E   Wang Ruonan R   Liu Liang L   Liu Jiawen J   Yuan Hongli H  

International journal of molecular sciences 20200107 2


Fermentable sugars are important intermediate products in the conversion of lignocellulosic biomass to biofuels and other value-added bio-products. The main bottlenecks limiting the production of fermentable sugars from lignocellulosic biomass are the high cost and the low saccharification efficiency of degradation enzymes. Herein, we report the secretome of <i>Trichoderma harzianum</i> EM0925 under induction of lignocellulose. Numerously and quantitatively balanced cellulases and hemicellulases  ...[more]

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