Ontology highlight
ABSTRACT:
SUBMITTER: Eller JM
PROVIDER: S-EPMC6422705 | biostudies-literature | 2019 Apr
REPOSITORIES: biostudies-literature
Eller Jared M JM Stewart Melissa L ML Slepian Alexandria J AJ Markwardt Sheila S Wiedrick Jack J Cohen Michael S MS Goodman Richard H RH Cambronne Xiaolu A XA
Current protocols in cytometry 20181217 1
Flow cytometry approaches combined with a genetically encoded targeted fluorescent biosensor are used to determine the subcellular compartmental availability of the oxidized form of nicotinamide adenine dinucleotide (NAD<sup>+</sup> ). The availability of free NAD<sup>+</sup> can affect the activities of NAD<sup>+</sup> -consuming enzymes such as sirtuin, PARP/ARTD, and cyclic ADPR-hydrolase family members. Many methods for measuring the NAD<sup>+</sup> available to these enzymes are limited bec ...[more]