Ontology highlight
ABSTRACT:
SUBMITTER: Hansen SR
PROVIDER: S-EPMC6430687 | biostudies-literature | 2019 Mar
REPOSITORIES: biostudies-literature
Hansen Sarah R SR Nikolai Brandon J BJ Spreacker Peyton J PJ Carrocci Tucker J TJ Hoskins Aaron A AA
Cell chemical biology 20190110 3
The spliceosome mediates precursor mRNA splicing in eukaryotes, including the model organism Saccharomyces cerevisiae (yeast). Despite decades of study, no chemical inhibitors of yeast splicing in vivo are available. We have developed a system to efficiently inhibit splicing and block proliferation in living yeast cells using compounds that target the human spliceosome protein SF3B1. Potent inhibition is observed in yeast expressing a chimeric protein containing portions of human SF3B1. However, ...[more]