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The PRP31 gene encodes a novel protein required for pre-mRNA splicing in Saccharomyces cerevisiae.

ABSTRACT: The pre-mRNA splicing factor Prp31p was identified in a screen of temperature-sensitive yeast strains for those exhibiting a splicing defect upon shift to the non- permissive temperature. The wild-type PRP31 gene was cloned and shown to be essential for cell viability. The PRP31 gene is predicted to encode a 60 kDa polypeptide. No similarities with other known splicing factors or motifs indicative of protein-protein or RNA-protein interaction domains are discernible in the predicted amino acid sequence. A PRP31 allele bearing a triple repeat of the hemagglutinin epitope has been generated. The tagged protein is functional in vivo and a single polypeptide species of the predicted size was detected by Western analysis with proteins from yeast cell extracts. Functional Prp31p is required for the processing of pre-mRNA species both in vivo and in vitro, indicating that the protein is directly involved in the splicing pathway.

SUBMITTER: Weidenhammer EM

PROVIDER: S-EPMC145753 | biostudies-other | 1996 Mar

REPOSITORIES: biostudies-other

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The PRP31 gene encodes a novel protein required for pre-mRNA splicing in Saccharomyces cerevisiae.

Weidenhammer E M EM Singh M M Ruiz-Noriega M M Woolford J L JL

Nucleic acids research 19960301 6

The pre-mRNA splicing factor Prp31p was identified in a screen of temperature-sensitive yeast strains for those exhibiting a splicing defect upon shift to the non- permissive temperature. The wild-type PRP31 gene was cloned and shown to be essential for cell viability. The PRP31 gene is predicted to encode a 60 kDa polypeptide. No similarities with other known splicing factors or motifs indicative of protein-protein or RNA-protein interaction domains are discernible in the predicted amino acid s ...[more]

PMID: 8604353

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