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RNAs in the spliceosome: Insight from cryoEM structures.

ABSTRACT: Pre-mRNA splicing is catalyzed by the spliceosome, a multimegadalton RNA-protein complex. The spliceosome undergoes dramatic compositional and conformational changes through the splicing cycle, forming at least 10 distinct complexes. Recent high-resolution cryoEM structures of various spliceosomal complexes revealed unprecedented details of this large molecular machine. This review highlights insight into the structure and function of the spliceosomal RNA components obtained from these new structures, with a focus on the yeast spliceosome. This article is categorized under: RNA Processing > Splicing Mechanisms RNA Structure and Dynamics > RNA Structure, Dynamics, and Chemistry RNA Interactions with Proteins and Other Molecules > RNA-Protein Complexes.

SUBMITTER: Zhang L

PROVIDER: S-EPMC6450755 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature

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RNAs in the spliceosome: Insight from cryoEM structures.

Zhang Lingdi L Vielle Anne A Espinosa Sara S Zhao Rui R

Wiley interdisciplinary reviews. RNA 20190206 3

Pre-mRNA splicing is catalyzed by the spliceosome, a multimegadalton RNA-protein complex. The spliceosome undergoes dramatic compositional and conformational changes through the splicing cycle, forming at least 10 distinct complexes. Recent high-resolution cryoEM structures of various spliceosomal complexes revealed unprecedented details of this large molecular machine. This review highlights insight into the structure and function of the spliceosomal RNA components obtained from these new struc ...[more]

PMID: 30729694

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