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Experimental and Computational Protocols for Studies of Cross-Seeding Amyloid Assemblies.

ABSTRACT: Alzheimer's disease (AD) and type 2 diabetes (T2D) are two common protein aggregation diseases. Compelling evidence has shown a link between AD and T2D, which may derive from interspecies cross-sequence interactions between amyloid-? peptide (A?), associated with AD, and human islet amyloid polypeptide (hIAPP), associated with T2D. Herein, we present experimental and computational protocols and tools to study the aggregate structures and kinetics, conformational conversion, and molecular interactions of A?-hIAPP mixtures. These protocols could be generally applied to other cross-seeding behaviors of amyloid peptides.

SUBMITTER: Ren B

PROVIDER: S-EPMC6456059 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature

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Experimental and Computational Protocols for Studies of Cross-Seeding Amyloid Assemblies.

Ren Baiping B Hu Rundong R Zhang Mingzhen M Liu Yonglan Y Xu Lijian L Jiang Binbo B Ma Jie J Ma Buyong B Nussinov Ruth R Zheng Jie J

Methods in molecular biology (Clifton, N.J.) 20180101

Alzheimer's disease (AD) and type 2 diabetes (T2D) are two common protein aggregation diseases. Compelling evidence has shown a link between AD and T2D, which may derive from interspecies cross-sequence interactions between amyloid-β peptide (Aβ), associated with AD, and human islet amyloid polypeptide (hIAPP), associated with T2D. Herein, we present experimental and computational protocols and tools to study the aggregate structures and kinetics, conformational conversion, and molecular interac ...[more]

PMID: 29744852

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