Dataset Information

Draft Genome Sequence of Lecanicillium sp. Isolate LEC01, a Fungus Capable of Hydrocarbon Degradation.

ABSTRACT: Lecanicillium sp. isolate LEC01 is adapted to grow in the presence of jet fuel, employing genes involved in the degradation of alkanes and aromatic hydrocarbons. The draft genome is estimated at 31,407,988?bp and has 9,737 proteins, 50.0% G+C content, and high similarity to Lecanicillium sp. strain CCF 5233.

SUBMITTER: Radwan O

PROVIDER: S-EPMC6460043 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature

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Draft Genome Sequence of Lecanicillium sp. Isolate LEC01, a Fungus Capable of Hydrocarbon Degradation.

Radwan Osman O Gunasekera Thusitha S TS Ruiz Oscar N ON

Microbiology resource announcements 20190411 15

Lecanicillium sp. isolate LEC01 is adapted to grow in the presence of jet fuel, employing genes involved in the degradation of alkanes and aromatic hydrocarbons. The draft genome is estimated at 31,407,988 bp and has 9,737 proteins, 50.0% G+C content, and high similarity to Lecanicillium sp. strain CCF 5233. ...[more]

PMID: 30975820

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Project description:BackgroundIn our continuing search for biologically active natural enemies from North of Africa with special reference to Tunisian fungi, our teamwork screened fungi from different ecological habitats in Tunisia. Our previous study on the comparative effectiveness of filamentous fungi in the biocontrol of Meloidogyne javanica, a taxon (Lecanicillium) showed high potentiality against M. javanica. We undertook the present study to evaluate the ability and understand the mechanism of this fungal parasite as a biological control candidate against the root-knot nematode M. javanica. This study used in vitro bioassays with fungal filtrate cultures, scanning electron microscopy (SEM) observation, and isobaric tag for relative and absolute quantitation (iTRAQ) methodology to characterize the biological and molecular features of this fungus.ResultsThe microscopic and SEM observation revealed that Lecanicillium sp. exhibited exceptional hyperparasitism against M. javanica eggs. The hyphae of this fungi penetrated the eggs, causing destructive damage to the outer eggshell. The exposure to five concentrations of Lecanicillium sp. filtrate cultures showed high inhibition of egg hatching, which increases depending on the exposure time; the best results are recorded at 50%, 75%, and 100% dilutions after seven days of exposure. The SEM observation of nematode-parasitized eggs and juveniles suggests that the production of lytic enzymes degrades the egg cuticle and fungal hyphae penetrate unhatched M.javanica juveniles. Forty-seven unique proteins were identified from the Lecanicillium sp. isolate. These proteins have signalling and stress response functions, bioenergy, metabolism, and protein synthesis and degradation.ConclusionCollectively, Lecanicillium sp. had ovicidal potentiality proved by SEM and proteomic analysis against root-knot nematode' eggs. This study recommended applying this biological control candidate as a bio-agent on vegetable crops grown in situ.

Dataset Information

Draft Genome Sequence of Lecanicillium sp. Isolate LEC01, a Fungus Capable of Hydrocarbon Degradation.

Publications

Draft Genome Sequence of <i>Lecanicillium</i> sp. Isolate LEC01, a Fungus Capable of Hydrocarbon Degradation.

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