Unknown

Dataset Information

0

Endocytosis Pathways of Endothelial Cell Derived Exosomes.


ABSTRACT: Nanosized extracellular vesicles (EVs) possess the natural machinery needed to enter selectively and transmit complex molecular messages efficiently into targeted cells. The intracellular fate of the vesicular cargos depends on the route of internalization. Therefore, understanding the mechanism of attachment and subsequent intake of these vesicles (before and after exerting any modification) is imperative. Here the extent of communication, the uptake kinetics, and the pathways of endothelial EVs into endothelial cells in the presence of specific pharmacological inhibitors were assessed by imaging flow cytometry. The results showed that the uptake of endothelial EVs into endothelial cells was largely an energy-dependent process using predominantly a receptor-mediated, clathrin-dependent pathway.

SUBMITTER: Banizs AB 

PROVIDER: S-EPMC6465166 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature

altmetric image

Publications

Endocytosis Pathways of Endothelial Cell Derived Exosomes.

Banizs Anna B AB   Huang Tao T   Nakamoto Robert K RK   Shi Weibin W   He Jiang J  

Molecular pharmaceutics 20181030 12


Nanosized extracellular vesicles (EVs) possess the natural machinery needed to enter selectively and transmit complex molecular messages efficiently into targeted cells. The intracellular fate of the vesicular cargos depends on the route of internalization. Therefore, understanding the mechanism of attachment and subsequent intake of these vesicles (before and after exerting any modification) is imperative. Here the extent of communication, the uptake kinetics, and the pathways of endothelial EV  ...[more]

Similar Datasets

| S-EPMC7686085 | biostudies-literature
| S-EPMC4274510 | biostudies-other
| S-EPMC6806135 | biostudies-literature
| S-EPMC5689125 | biostudies-other
| S-EPMC5503715 | biostudies-literature
| S-EPMC9990300 | biostudies-literature
| S-EPMC4991413 | biostudies-literature
| S-EPMC10791899 | biostudies-literature
| S-EPMC3316594 | biostudies-literature
| S-EPMC2674948 | biostudies-literature