Unknown

Dataset Information

0

Structural insight into YcbB-mediated beta-lactam resistance in Escherichia coli.


ABSTRACT: The bacterial cell wall plays a crucial role in viability and is an important drug target. In Escherichia coli, the peptidoglycan crosslinking reaction to form the cell wall is primarily carried out by penicillin-binding proteins that catalyse D,D-transpeptidase activity. However, an alternate crosslinking mechanism involving the L,D-transpeptidase YcbB can lead to bypass of D,D-transpeptidation and beta-lactam resistance. Here, we show that the crystallographic structure of YcbB consists of a conserved L,D-transpeptidase catalytic domain decorated with a subdomain on the dynamic substrate capping loop, peptidoglycan-binding and large scaffolding domains. Meropenem acylation of YcbB gives insight into the mode of inhibition by carbapenems, the singular antibiotic class with significant activity against L,D-transpeptidases. We also report the structure of PBP5-meropenem to compare interactions mediating inhibition. Additionally, we probe the interaction network of this pathway and assay beta-lactam resistance in vivo. Our results provide structural insights into the mechanism of action and the inhibition of L,D-transpeptidation, and into YcbB-mediated antibiotic resistance.

SUBMITTER: Caveney NA 

PROVIDER: S-EPMC6478713 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature

altmetric image

Publications


The bacterial cell wall plays a crucial role in viability and is an important drug target. In Escherichia coli, the peptidoglycan crosslinking reaction to form the cell wall is primarily carried out by penicillin-binding proteins that catalyse D,D-transpeptidase activity. However, an alternate crosslinking mechanism involving the L,D-transpeptidase YcbB can lead to bypass of D,D-transpeptidation and beta-lactam resistance. Here, we show that the crystallographic structure of YcbB consists of a c  ...[more]

Similar Datasets

| S-EPMC5089857 | biostudies-literature
| S-EPMC11347637 | biostudies-literature
| S-EPMC9603335 | biostudies-literature
| S-EPMC2775221 | biostudies-literature
| S-EPMC4353011 | biostudies-literature
| S-EPMC8633438 | biostudies-literature
| S-EPMC8773433 | biostudies-literature
| S-EPMC6884583 | biostudies-literature
| S-EPMC4401780 | biostudies-literature
| S-EPMC3917139 | biostudies-literature