Relative proteome quantification of alpha, beta, gamma and delta globin chains in early eluting peaks of Bio-Rad variant II^® CE-HPLC of hemoglobin from healthy and beta-thalassemia subjects in Malaysia.

ABSTRACT: This is the first report of QQQ-mass spectrometric identification and quantification of the Hb subunits, alpha, beta, delta and gamma globin peptides, derived from enzymatic-digestion of proteins in the early unknown peaks of the Bio-Rad cation-exchange chromatography of haemoglobin. The objectives were to assess the relationship of the quantity of the free alpha, beta, delta and gamma globin chains with the phenotypic diversity of beta-thalassaemias (?-thal). The results demonstrate that the pools of free globin chains in red blood cells were correlating with the severity of the disease in patients with different phenotypes of ?-thal. The mechanism and the regulation of synthesis of free globin chains pool in a normal individual and in patients with different ?-thal phenotypes could arise from several mechanisms which will require further investigation. The role of the free globin pool in patients with ?-thal for development of novel therapeutic approaches based on these potential targets requires further investigation. Pertinent biomarkers improves the diagnosis of the ?-thal, especially in low-income countries where they are most common and allows more effective therapeutic intervention leading to more successful therapeutic outcome.

SUBMITTER: Abdullah UYH 

PROVIDER: S-EPMC6488526 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature