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Circulating Microparticles Decrease After Cardiac Stress in Patients With Significant Coronary Artery Stenosis.

ABSTRACT: BACKGROUND:Cardiac stress leads to a dynamic increase of circulating microparticles (MPs) in healthy individuals that is diminished in individuals with vascular disease. The impact of coronary ischemia on circulating MP level is unknown. This study investigates the kinetics of circulating MPs during cardiac stress in patients with coronary artery stenosis. HYPOTHESIS:Patients with significant coronary stenosis show altered circulating MP levels after cardiac stress. METHODS:Eighty patients with stable coronary artery disease underwent dobutamine stress echocardiography (DSE) on the day before coronary angiography. Before, immediately after, at 4 hours, and at 24 hours after DSE, blood was drawn to determine CD144+ endothelial microparticles (EMPs), CD14+ CD16+ monocyte-derived microparticles (MMPs), and CD31+ CD42b+ platelet microparticles. A significant stenosis was defined as stenosis diameter ?70% in a major native epicardial coronary artery with a diameter of ?2.5?mm. RESULTS:Significant coronary artery stenoses were found in 41 patients. In these patients, CD144+ -EMP and CD14+ CD16+ -MMP concentrations decreased immediately after DSE. Stimulation of target endothelial cells with sera from patients with significant coronary artery stenoses significantly augmented endothelial capacity to take up EMPs, but not MMPs, in vitro. Serum-induced enhancement of endothelial phosphatidylserine receptor expression was found as a potential mechanism of increased endothelial EMP uptake and subsequently reduced circulating EMP levels after cardiac stress. CONCLUSIONS:Cardiac ischemia leads to reduced circulating MP levels under cardiac stress. Changes of endothelial MP uptake capacities could be one possible mechanism.

SUBMITTER: Sinning JM

PROVIDER: S-EPMC6490784 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature

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Circulating Microparticles Decrease After Cardiac Stress in Patients With Significant Coronary Artery Stenosis.

Sinning Jan-Malte JM Jansen Felix F Hammerstingl Christoph C Meier Arne A Losch Jan J Rohwer Katharina K Schmitz Theresa T Paul Kathrin K Sedaghat Alexander A Schueler Robert R Vasa-Nicotera Mariuca M Müller Cornelius C Nickenig Georg G Werner Nikos N

Clinical cardiology 20160713 10

<h4>Background</h4>Cardiac stress leads to a dynamic increase of circulating microparticles (MPs) in healthy individuals that is diminished in individuals with vascular disease. The impact of coronary ischemia on circulating MP level is unknown. This study investigates the kinetics of circulating MPs during cardiac stress in patients with coronary artery stenosis.<h4>Hypothesis</h4>Patients with significant coronary stenosis show altered circulating MP levels after cardiac stress.<h4>Methods</h4 ...[more]

PMID: 27410166

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Project description:Monocytes and T-cells play an important role in the development of atherosclerotic coronary artery disease (CAD). Differences in transcriptional activity of these cells might reflect the individual's atherosclerotic burden. Transcriptome analysis of circulating mononuclear cells from carefully matched atherosclerotic and control patients will potentially provide insights into the pathophysiology of atherosclerosis and supply biomarkers for diagnostic purposes. From patients undergoing coronary angiography because of anginal symptoms, we carefully matched 18 patients with severe triple-vessel CAD to 13 control patients without signs of CAD on angiography. All patients were on statin and aspirin treatment. RNA from circulating CD4+ T-cells, CD14+ monocytes, lipopolysaccharide-stimulated monocytes, macrophages and CD34+ progenitor cells was subjected to genome-wide expression analysis. Only CD14+ monocytes demonstrated that a small number of genes involved in activation was overexpressed in control patients, which was verified by real-time polymerase-chain reaction. In this pilot study, cautious matching of patients with severe atherosclerotic CAD with control patients without angiographic signs of coronary atherosclerosis did not reveal differences in transcriptional activity in four out of five different mononuclear cell types. In resting monocytes from patients without overt CAD some inflammatory genes were overexpressed as compared to patients with severe CAD. Large inter-individual variability prevented the use of single differentially expressed genes as biomarkers. Keywords: disease-state analysis In total 153 arrays were analyzed with 6 technical replicates (147 biological samples). CD34+ stem cells, CD4+ T-cells, resting CD14+ monocytes, stimulated monocytes and macrophages were analyzed, all from patient with severe coronary atherosclerosis or controls that had no coronary atherosclerosis as determined angiographically, and which were carefully matched for age and gender.

Dataset Information

Circulating Microparticles Decrease After Cardiac Stress in Patients With Significant Coronary Artery Stenosis.

Publications

Circulating Microparticles Decrease After Cardiac Stress in Patients With Significant Coronary Artery Stenosis.

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